I have been studying DLL4 protein expression on HUVECs. But, every time my membrane shows many bands. I have been using a polyclonal antibody at appropriate dilutions. Is there any specific antibody that works for others? Thanks in advance
I suppose because it is a polyclonal antibody you are getting some non-specific binding of it to other random proteins. Are you seeing any evidence for this exact antibody working more cleanly elsewhere (and not evidence from the manufacturer website)?
Thats what I also thought. I didn’t find any online blot images with this antibody apart from the ones from the manufacturer. My colleagues at work have had better experiences with this antibody and I am just trying to rule out technical issues.
Thank you so much for your answer
No problem, blocking could be another thing to check, if your colleagues use a different blocking solution you could try that. If it is a very important antibody, remember you can cut your blot into strips post transfer and then treat each little strip as a mini blot, and perform your trouble shooting.
At the same time, always is a chance the antibody is just not going to work.
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