MCF-7 cells are transfected with caspase-3 Rluc 8.6 plasmid and the cells were treated with staurosporine drug in order to induce apoptosis. I see increased luciferase activity in apoptotic cells versus normal cells. What could be the possible reason for this?
Divaker Choubey
Additionally, the enzyme may be "released" from apoptotic cellular vesicles, thus, more active in the assay.
1 votes
1 thanks
Niklas Dienstbier
Most readouts need a soft lysis step prior to the actual readout to make the luciferase enzyme accessible for the reagent. If you are using such as non-homogeneous assay system, it could be possible that the apoptosis substitutes non-sufficient lysis and therefore increases the signal.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India
More Punita Bathla's questions See All
Similar questions and discussions