I was using the thin film hydration method to form the niosome using Span 60 and cholesterol (100:120 umol) dissolved in chloroform.
After evaporation under 60 oC and 100 rpm for one hour, i always got the inhomogeneous thin film with some white spots as shown in the figures. And it becomes difficult to hydrate those thin films and i would always get a lot white aggregates.
What can cause those problems? Is that because the chloroform is not stable and some reaction happened?
Hi,
At this stage I can suggest 2 possible solutions (if not solved your problems let me know and we find another way):
1. Use a solvent-free method for noisome preparation.
or:
2. Use another solvent or solvent mix (e.g. Chloroform and Methanol).
Thanks so much for your suggestion, i will try these methods and see how it goes
Hi Yuhang,
The concentrations you are using maybe higha ndnthe lipid recrystalillizes upon removal of solvent. The rate of solvent removal could also influence recrysatllization. As Prof Mozafari suggested using a solvent mix could help but ideally as the solvents evaporates recrystallization may be inevtable. Have you considered a method that removes solvent post niosome formation such as ether injection or reverse phase formation?
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