HI,
Iam using licor rabbit secondary antibody green color in the 800 channel and I see a lot of background for some of the primary antibodies, though some of them work perfectly. Is there any reason. Iam not using any IgG subtype specific antibodies. Same happens for donkey and goat, both of which I have tested. Has anyone faced the same problem?
Thanks
jaya
Steingrimur Stefansson
Hi Jaya, I only use secondary's from Jax labs, which have been immunodepleted. I don't know about licor. I have never heard of them.
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Vivica Grotelueschen
Sometimes components of the washing buffers have an influence on the background with Licor. You might try Tris-based buffers (TBS) instead of phosphate buffer (PBS) or leave out the detergents (Tween or Triton).
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