HIV may induce CD4+cell apoptosis or interfere with its function.
I am using TfOH, DMC, dimethyldimethoxysilane and MM as the starting material, I have tried to reduce the amount of MM, but I constantly obtained low viscosity silicone oil (~100 mPa`s). I once...
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In terms of the lattice parameters and miller indices? and how can I identify whether a material is heterojunction or heterostructure? and which characterization method is useful to know whether...
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Gene based primer construction
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Can triparental mating insert gfp segment from a plasmids into another plasmids?I read a research paper 《Plasmid-mediated bioaugmentation for the degradation of chlorpyrifos in soil》. i have some...
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For the human genome, do you explain this with probability or is it empirically determined?
01 September 2014 7,525 5 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
16 July 2024 7,475 1 View
Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...
13 July 2024 356 3 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
08 July 2024 5,482 2 View
Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...
02 July 2024 4,887 0 View
Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...
01 July 2024 8,964 1 View
Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...
27 June 2024 7,707 2 View
My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...
10 June 2024 6,253 0 View
