How to obtain covariance function in a binary digitalized image?

Hello every one, Does anybody know how we can practically obtain the covariance function C(X,h) of a binary image? Best regards, Pedram

05 January 2021 8,722 3 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

Is it possible for anybody to help me for fix bridge modeling in OPENSEES?

I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...

12 July 2020 415 3 View

No title

14 July 2019 6,848 5 View

How to calculate the Curie temperature in thermo-calc software?

The curie temperature is a temperature which above it the magnetization of ferrite/martensite disappears in steel, how we can calculate this temperature via thermo-calc software?

25 January 2017 4,793 3 View

Which Eyetracker is good for working with children?

I want to know that is there any special eyetracker device model that can be used for working with children between 6-24 months. There is many models from companies like SMI, Tobii, Eyelink, ...,...

16 September 2016 9,041 1 View

Underground water finder tool?

Hello, I am Phd candidate and I want to study on environmental factors influencing Tugai forests (riparian forests of dry regions) located at Khouzestan province, Iran. I am looking for an...

14 February 2016 231 2 View

I have trouble in detecting my protein of interest in total cancer cell line lysate in western blot. How should I solve this problem?

I can detect beta actin band and my cell line is MDA-MB-231 breast cancer cell line.

29 September 2015 7,619 20 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View