Why DNase I does not cut ~ 200bp fragment next to the labelled FAM ?

I am trying to set up for DNase I footprinting. I use 50ng of PCR product (app. 500 bp) labelled with 5' FAM. I am using RQ1 enzyme from promega. I am using 0.1U of DNase I with incubation on...

26 July 2021 4,389 0 View