I think you meant BEFORE using the filter for your sample. If you refer to the syringe filter manufacturers various manuals and website information, you will find that most suggest you wet the filter with the solution first, before injecting your sample through them. The various membranes have a hold-up (abs) volume which must first be meet. Washing the filter first, then injecting your sample through the filter allows the filter to do its job more efficiently.
I think you meant BEFORE using the filter for your sample. If you refer to the syringe filter manufacturers various manuals and website information, you will find that most suggest you wet the filter with the solution first, before injecting your sample through them. The various membranes have a hold-up (abs) volume which must first be meet. Washing the filter first, then injecting your sample through the filter allows the filter to do its job more efficiently.
This linked article may also be of interest to you.
"Syringe Filter Selection for HPLC or LC/MS samples"
http://hplctips.blogspot.com/2014/11/syringe-filter-selection-for-hplc-or.html
You can either follow it or do a simple experiment. Don't discard a few mL sample, filter and continuously collect 10 samples (1 mL per one). My concern is that the filter might have minor adsorption of your analytes, which might reduce your signal 1-5% if you don't discard any sample. Filter study is required in some method validations.
- Badges
- Science method