Dear all researchers, I am culturing Bifidobacterium sp. in MRS broth, however, abnormal growing pattern is observed. All this while Bifidobacterium will only form a sediment at the bottom of MRS broth with clear supernatant after 3 days incubation, however recently I found out that an uniform turbidity is formed in the supernatant (as attached image). Does anyone experience it before? I have did some testing and the turbid layer is not bacteria cells. Tq.
Hi Cristiano,
Thanks for your answer. I am suspecting it was organic waste as well. I will consider your suggestion. Tq.
Bifidobacterium are known to produce significant amount of bacterial exopolysaccharides (EPS) during their growth towards the late log and stationary phase...these turbidity in the supernatent could be due to that too...can read:
Appl Environ Microbiol. 2014 Jan;80(1):9-18. doi: 10.1128/AEM.02977-13. Epub 2013 Oct 11. Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp.http://www.ncbi.nlm.nih.gov/pubmed/24123746
there are simple biochemical assays to confirm EPS production, but detailed analyses of the sugars and linkages may require a MS or NMR.. good luck!
Hi Dave, Thanks for your answer, when the uniform turbidity form in MRS broth, it will causing the cells dead and unable to growth in milk. It looks strange, unless the "waste" that produced by them will kill back themselves. Cheers.
Hi, do you think the excessive exopolysaccarides will kill bifido? Thanks.
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