11 Questions 9 Answers 0 Followers
Questions related from Sherlyn Tang
Dear all researchers, Can I count the bacterial cells using hemocytometer under light microscope? Which staining dye can I use? Can I use crystal violet or trypan blue? What percentage and under...
05 April 2016 815 10 View
Dear all researchers, I was facing a problem during subculturing L. gasseri and L. fermentum in MRS broth. After 3 days incubation, the broth itself become cloudy and sticky. Gasses was formed and...
21 March 2016 7,437 5 View
Dear all researchers, can anyone provide me a salting out protocol for butyric acid extraction? Tq.
04 January 2016 1,491 8 View
Is there any Gram-negative bacteria is unable to growth on MacConkey agar, EMB agar and Salmonella Shigella Agar but form colony on TSA and MRSA? Based on gram stain, it is pink in color and in...
11 September 2015 7,599 4 View
Dear all researchers, is there any method can reactivate the dormant cells of Lactobacillus? Thanks.
22 July 2015 7,904 8 View
Dear all researchers, I am culturing Bifidobacterium sp. in MRS broth, however, abnormal growing pattern is observed. All this while Bifidobacterium will only form a sediment at the bottom of MRS...
22 June 2015 3,629 5 View
I'm not a chemist and does not know much about the theory behind the zinc titration method. I have a sample and wold like to test for the zinc content. Following is the protocol that I have...
20 October 2014 8,540 4 View
Dear researchers, Recently, I have been facing mysterious test results. I was collecting samples of probiotic cultures from a large fermenter tank into 100mL lab bottle under sterile conditions,...
15 October 2014 6,204 3 View
May I know is there an alternative drying method that can be used to replace spray dryer? The sample used will be encapsulated bacterial cells.
03 March 2014 2,085 1 View
I would like to seek your advice towards the strange result that I have obtained during my microencapsulation process. I was preparing two samples as follows: 1. Add fructo-oligosaccharide...
19 February 2014 9,870 1 View
I am looking for a protocol for total bacteria count, which include both viable and non-viable cells. The bacteria that I focus on are probiotic. Anyone have any advice?
14 February 2014 4,983 10 View
