10 February 2015 0 1K Report

I am trying to screen antibiotic disc using disc diffusion method so I have prepared the inoculum of P. acnes as the following

The 72 h culture of the bacteria was dissolved in .85% saline and the suspension was adjusted to 0.5 McFarland standard turbidity. A sterilized cotton swab was

dipped in the P. acnes inoculum and agar plate was streaked 4 times and I also subculture some plate. 

I repeated the experiment two times, but still no lawn form. I only got a result for my subculture plate

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