what could be the possible reason for poor gel image after running a pcr product. please find the attached image?
I suppose that the first line is the marker; so, one of the reason why you get such a "poor" gel image, shoud be the method used for staining. Can you specify how you performed it?
I think you should check your gel preparation formulation depending on the percentage of agarose and the amount of the dye e.g Ethidium bromide . For example if you use 2g of agarose to 100ml 1X TBE buffer increase your agarose percentage to reach 3% .
I think the problem with the buffer used in gel electrophoresis run, may be prepared a long time ago. Try to change the buffer with a new one (1X TAE or TBE)
Dear, Carolina Chiellini
yes the first lane is 100bp ladder. In our case we didn't stain and dis stain the gel rather we add 2 micro litter EtBr in to aggarose-1x TBE buffer mix and load the samples. we use 2% agarose (2g into 100ml1x TBE buffe).
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