Dear Scientists,
I am working with a gene of interest in colorectal cancer, which has no role in cell proliferation. In addition, by knockdown this gene, the FAK and SRC protein expression are decreased. I have changed amino acid by SDM and check the protein expression of the gen by WB, the expression is decreased and the MW was less than actual MW. Then, I check the cell proliferation after transfection with mutated plasmid and wild type plasmid, I have noticed a significant increase in proliferation of the mutated one compare to WT. Can anyone have an idea to explain how this is happening?
Thank you in advance,
It may or may not help to answer the question, but what is the normal role of the [WT] gene or protein if it is not involved in proliferation?
Hi Brandon,
This gene play a major role in promoting cancer cell invasion and migration, but not proliferation. In addition, it has role in binding of colorectal cancer cells to endothelial cells, which eventually leads to cancer metastasis.
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