A colleague who works with p53 needs to quantify this protein by Western Blot. He tried it in several ways, but he never could see the protein in the membrane. After checking for many possible protocol errors and antibody problems, he stained the gel with coumassie and did not check any band on the gel except once with the approximate size of p53. How can this happen?
Marília Figueira
Thank you for your answer. We observe the band in the gel after protein transfer.
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