Marília Figueira

2 Questions 3 Answers 0 Followers

Questions related from Marília Figueira

Why can proteins with specific size stay in the acrylamide gel and are not transferred to the PVDF membrane in Western blot?

A colleague who works with p53 needs to quantify this protein by Western Blot. He tried it in several ways, but he never could see the protein in the membrane. After checking for many possible...

07 July 2018 7,020 1 View

Has anyone made electrotransference in Transblot Turbo equipment of BioRad using acrylamide gels 1.5mm?

Hi, we tried to transfer proteins from an acrylamide gel 1.5mm made with our recipe to a PVDF membrane of BioRad in the Transblot Turbo of BioRad, but the majority of protein rested in the gel....

02 February 2015 9,065 5 View