We used heparin as an anticoagulant during blood sampling from fish. Then used 5000Xg for 10 minutes to separate then plasma. We stored the plasma in -80C. But when I take out the plasma sample for analysis and keep it on ice for melting it becomes jelly-like does not become liquid. Some of the samples are half liquid, half jelly. Can anyone suggest me, what might be the reasons for such situations and how to improve it?
Thank you Dr Emmanuel Ifeanyi Obeagu for your answer. Do you have any suggestion on how to resolve this problem?
Plasma is a mixture of protein , lipid ,mineral ,water etc. and have high viscosity so it is a jelly like substance. Ingredient in it is not soluble in water.
Thank you for your answer Dr Dharmesh Chandra Sharma. So you are saying it's normal to become plasma as jelly, right?
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue