Syed Monzur Morshed

10 Questions 33 Answers 0 Followers

Questions related from Syed Monzur Morshed

How to clean pipette before qPCR experiment?

I have seen that people suggest cleaning the pipette before doing the qPCR experiment. We use 0.2% NaOH + 0.4% SDS solution to clean bench to remove RNAase. So, to clean the pipette I first used...

03 May 2021 2,937 13 View

How can I know which sequence is the correct one if there are lot of predicted sequence in database for my target gene?

Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...

24 December 2020 5,708 12 View

Is it OK to use autoclaved double distilled water or DEPC water for qPCR experiment?

I was using molecular grade water for the qPCR experiment. I want to know if I use autoclaved double distilled water is it fine? What about using DEPC water? Please share your knowledge and...

14 December 2020 3,368 14 View

Is it OK to have same melting curve and same product size in gel run for NTC in qPCR experiment?

After doing my primer efficiency, my lowest dilution cDNA ct value is 20.16 and NTC ct value is 31.76. The melting curve is the same for target product and NTC and it is giving the same product...

09 December 2020 5,424 6 View

What is singletons in microbiota data?

I have seen in papers that many papers remove singletons from their microbiota data. I am not sure what is actually singletons and why the authors remove the singletons sequences. I would be happy...

24 November 2020 4,819 6 View

Should I vortex during qPCR solution mixing?

To do qPCR we have to mix solution or do serial dilution. Like, 1. cDNA dilution 2. Primer dilution 3. mixing SYBR Green, cDNA and Primer in PCR tube. My question is for doing this mixing when...

26 October 2020 2,694 11 View

What do you think about the RNA quality of the samples in this gel image?

I have run my RNA sample in 1% agarose gel. 28s and 18s band are not that much strong. I want suggestions so that I can know if the RNA quality is good for my sample or not.

24 September 2020 1,062 20 View

Why blood plasma is becoming as jelly like substance?

We used heparin as an anticoagulant during blood sampling from fish. Then used 5000Xg for 10 minutes to separate then plasma. We stored the plasma in -80C. But when I take out the plasma sample...

22 August 2020 3,687 4 View

How to make probiotic in different concentration?

I want to know how to make probiotic in different concentration. I am planning to probiotic experiment with different concentration. I also want to know how to make the freeze dry probiotic powder...

15 April 2020 1,314 2 View

Microbiome DNA extracted from fish feces giving weird result

Hello everyone, I have extracted microbiome DNA using the Qiamp Powersoil pro kit. After extracting DNA I used nanodrop to check the quality. I used the same last solution present in the DNA to...

01 January 1970 8,625 2 View