Dear all,
I am using Stempro-34 medium and with the requirement of 10^(-4) M Ascorbic acid addtion. I have my 0.1 M Ascorbic acid stock in DMSO. The solution was clear at room temperature. However, the moment when I add the appropriate amount of Ascorbic acid stock into the medium, the precipitation appears. There is nothing else added into the medium. Does anyone know how this happens? What can I do to get prevent the precipitation? Or it doesn't affect any active component in the medium, then I just ignore it?
Thanks for your help in advance!
AA has a solubility of 300g/L in water. Why would you dissolve it in DMSO? Just use your regular buffer/water.
What kind of medium are you using?
And precipitate in slow addition of methanol. As you start addition of methanol into water soltion of ascorbic acid, it will start precipitating and take 5th 7 times methanol then water you have added. You will get pets of ascorbic acid.
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