Zhao Yuling

3 Questions 1 Answers 0 Followers

Questions related from Zhao Yuling

Why Ascorbic acid precipitates in cell culture medium?

Dear all, I am using Stempro-34 medium and with the requirement of 10^(-4) M Ascorbic acid addtion. I have my 0.1 M Ascorbic acid stock in DMSO. The solution was clear at room temperature....

31 March 2022 6,772 2 View

What are the pallets in my in vitro phosphorylation reaction?

My aim is to phosphorylate the proteins (produced by rabbit reticulocyte lysate) via a specific kinase and then remove the phosphorylation via lambda phosphatase (LP). The problem is before LP...

12 November 2020 4,606 2 View

Why my stack gel of BN-PAGE can't polymerize?

Hi, everyone! I am really confused. From yesterday, my stack gel of BN-PAGE never polymerize. However, the same materials I used for making the running gels, which polymerize normally. I was...

03 October 2020 3,362 1 View