Why are the samples of protein getting very viscous and it's becoming very hard to load the sample in SDS-PAGE?

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Ekaterina Gracheva

Hi Amit,

How do you prepare your samples and what kind of cells do you use?

M Rohan Fernando

It is because of high nucleic acid concentration in your sample. You can treat your sample with a nuclease for 10 - 15 min. In my experience heating to 80 - 90 0C may also help.

Amit Kumar Singh

Dear

Ekaterina

I am using DT40 chicken cells

and taking 2 million cells in 30 ul of 1 X PBS and then adding to 30 ul of 2X sample buffer consisting of b mercaptoethanol

After that heating at 95 degrees