I am using 30 ul of 2X sample buffer for 2 million cells in 30 ul 1X PBS
But, my sample is getting very viscous and it is very hard to load it in wells
every time samples comes out from wells
So, please suggest me the reasons for this
Ekaterina Gracheva
Hi Amit,
How do you prepare your samples and what kind of cells do you use?
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M Rohan Fernando
It is because of high nucleic acid concentration in your sample. You can treat your sample with a nuclease for 10 - 15 min. In my experience heating to 80 - 90 0C may also help.
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Amit Kumar Singh
Dear
Ekaterina
I am using DT40 chicken cells
and taking 2 million cells in 30 ul of 1 X PBS and then adding to 30 ul of 2X sample buffer consisting of b mercaptoethanol
After that heating at 95 degrees
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