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Currently I am expressing my protein in Rosetta cells and planning to purify the protein. Can anybody please suggest me the detailed procedure to generate antibodies against a protein in lab?
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Why there are more dead cells are present after one week of reviving? I centrifuge the cells at low RPM and then wash with PBS and add fresh media to it but the no. of dead cells remain same? Can...
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I am using 30 ul of 2X sample buffer for 2 million cells in 30 ul 1X PBS But, my sample is getting very viscous and it is very hard to load it in wells every time samples comes out from wells So,...
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