Strawberry direct shoot regeneration on media containing TDZ+IBA and then on multiplication media containing BAP+IBA. After one week these plantlets show a reddish colour (shown in photo).
Nothing to worry. It has nothing to do with propagation medium. This is the plant’s reaction on the stress. Mostly these stress conditions are associated with prolonged irradiation of the plantlets. The red color you see is attributed to anthocyanin accumulation in the shoots or leaves.
I believe there must be related to stress due to the artificial media. The plantlet otherwise look healthy as you can see. Maybe it is due to the transference of plant tissue from one media to the next..
The presence of red color in the explants is a stress symptom. It is a negative situation when the plantlets reduce elongation o rooting. If the color is maintained in time, causing a slow development o differentiation is necessary to prevent it using adequate strategies. However, if the number of cases or the damage is scarce, may be is more easier eliminated the red part of explants. Sometimes, during the in vitro manipulation if the temperature o dryness is not controlled the explants can be stressed. In general, is convenient to manipulate the material using temperature and lighting similar to in vitro chamber.
Hi, Prakash, How much BAP/IBA have you used? The red color of the stem is a reaction on high concentration of the BAP/IBA. You also can optimize you culture medium composition to get less red color.
Good luck!
Besides stresses, which usually are not the case in tissue culture conditions, please also consider that there are some papers showing that cytokinins (TDZ, BAP, etc) are positive regulators of anthocyanin accumulation.
What was the light intensity in your conditions? How much sugar is in the medium? It is well-know that high light intensity in the presence of sugar may lead to antocyanin accumulations. http://www.plantphysiol.org/content/140/2/637.abstract
I do not think this red color is stress, hormones or sugar. The plantlets of all varieties of the yam species (Dioscorea alata) in my lab are always red in culture. They turn back green when they are hardened, no problem. I think it is more due to the low photosynthetic nature of tissue culture. Just like in some plants, the new leaves are red before they expand and become green.
- Badges
- Science topic
- Similar topics
- Philosophy
- Ethics