In most physiological phosphorylation reactions ATP and GTP are preferred over UTP, CTP or TTP. Why?
I am starting a membrane vesicle assay shortly. As I am doing it for the first time I don't know which filter to use for rapid filtration. The source of vesicles is HEK293 & CHO Cells....
05 June 2017 4,968 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...
15 July 2024 1,940 3 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I was performing a site directed mutagenesis of a plasmid, the goal was to change/insert 4 nucleotides into the original plasmid using primers as seen in the image: Now I now there may be some...
04 July 2024 5,221 2 View
No response from fish physiology and biochemistry journal after a year of peer review. Journal editorial team is not responding even for Request for withdrawal of our manuscript. We are struck...
25 June 2024 8,918 0 View