Hi Mehrajuddin, my guess is that bull semen preservation is more commercially important and thus has received more money to perfect a cryopreservation protocol.

Dear Mehrajuddin

There are many physiological differences between a male reproductive system in mammals and birds, besides differences in sperm ability and seminal plasma components.

Please, read in the following link:

https://www.researchgate.net/publication/270287013_COMPARISON_OF_THE_EFFECT_OF_VARIOUS_CRYOPROTECTIVE_AGENTS_ON_BIOCHEMICAL_TRAITS_OF_IRAQI_ROOSTERS_SEMEN

and

https://www.ncbi.nlm.nih.gov/pubmed/16523619

Good Luck

Article COMPARISON OF THE EFFECT OF VARIOUS CRYOPROTECTIVE AGENTS ON...

The high poly unsaturated fatty acid in avian sperm membrane  is responsible  for it.

• Avain semen is very,very fragile outside  once it is collected artificially and has a very short span of life.  Perhaps, physiological differences in thermo-regulation and diurnal variations, climatic changes are quite different in avian and other bovine species require different methodologies in cryo- preservation. Please go through relevant literature as suggested by other scientists in this column.
• thanks

The colleagues gave great contributions here. I recommend the studies on amides as cryoprotectants. Please refer to: Article Pellet Method of Semen Cryopreservation: Effect of Cryoprote...

Density of avian spermatozoa in terms of sperms to accessary fluids is very much different compared to bovine semen and hence, liveability/survival rate outside the bird's body temp. when exposed to outside environment with or without insulation precautions is very low. Extreme shock during cryo preservation is another factor for survival. one may attempt to dilute avian semen with tender coconut water (milk) which is slightly pre-warmed sufficiently to enhance survival rate before subjecting to cryo preservation.

lnathgubbi

- The cylindrical-shaped head of cock spermatozoa and unique lipid composition causes plasma membrane damage during cryopreservation.

- Glycerol is the cryoprotectant used in cryopreservation and its large molecular weight causes low permeability. glycerol needs to be removed after cryopreservation and this is costly, difficult, and work-intensive.

- osmotic intolerance of chicken spermatozoa

- loss of lipid from membrane and cause damage to the membrane

- expensive to make more number of straw with very less quantity of semen