Semen gradient centrifugation and sperm motility.
Hi,
For producing density gradients for separation of cells and
subcellular components by centrifugation or by sedimentation at unit gravity.
Neutral, highly-branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/ml can be achieved.
Better osmotic properties than sucrose. Preserves functional and morphological integrity.
Useful for separating cells that are sensitive to centrifugation
and for separating cells of similar density but different size
(under conditions of sedimentation at unit gravity).
Does not penetrate biological membranes.
Serves as the raw material for preparation of Ficoll-Paque gradients.
Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
If you need technical note donot be hesitate to contact me
Good luck
Thank you all for valuable answers
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