I am looking at several protocols where people use BOTH EDTA and EGTA in solution. E.g.
10 mM Tris–HCL, pH8.0
200 mM NaCl
1 mM EDTA
0.5 mM EGTA
can anyone explain why? They seem to be doing the same ion chelation though, arent they?
I am doing an assay for interaction with a substrate and I need a detergent that will interfere with the enzymatic activity as little as possible. What is the general recommendation in this case....
10 November 2019 10,008 6 View
I am doing some his-tagged protein purification and I would like to see if the chromatography works by running a standard. Is there a cheap histidine tagged protein people tend to use? Also the...
03 April 2019 2,633 5 View
I have a peptide I would like to quantify with 214 nm abrobance. Is there an algorithm (or a rule of thumb,) to predict what the absorbance should be like there for 280nm?
03 April 2019 7,329 1 View
I need to get a filter of good porosity (flows well) at the bottom of a 200ul tip. I need to filter small volumes of solutions in 96 format. Any ideas? The filter needs to be inert (so C18 or C8...
31 December 2018 6,984 4 View
I would like to test the efficiency of desalting by a gel filtration column and I decided to check the desalting efficiency by monitoring removal of a dye through absorbance. Can anyone recommend...
01 February 2018 2,960 2 View
I have 500ul of sample from where I try to precipitate proteins.The sample is 2% SDS and very dilute. The conventional chl-methanol protocol suggests that I use 1:3:1:4 volumes...
03 April 2017 8,250 1 View
I need to perform offline fractionation by HILIC. I have done offline fractionation using disposable RP columns (I have used home made columns with oligo R3 material, but Thermo sells disposable...
10 November 2016 4,253 1 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,500 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View