8 Questions 6 Answers 0 Followers
Questions related from Pavel V Shliaha
I am doing an assay for interaction with a substrate and I need a detergent that will interfere with the enzymatic activity as little as possible. What is the general recommendation in this case....
11 November 2019 9,986 6 View
I am looking at several protocols where people use BOTH EDTA and EGTA in solution. E.g. 10 mM Tris–HCL, pH8.0 200 mM NaCl 1 mM EDTA 0.5 mM EGTA can anyone explain why? They seem to be doing...
06 June 2019 5,514 0 View
I am doing some his-tagged protein purification and I would like to see if the chromatography works by running a standard. Is there a cheap histidine tagged protein people tend to use? Also the...
04 April 2019 2,611 5 View
I have a peptide I would like to quantify with 214 nm abrobance. Is there an algorithm (or a rule of thumb,) to predict what the absorbance should be like there for 280nm?
04 April 2019 7,299 1 View
I need to get a filter of good porosity (flows well) at the bottom of a 200ul tip. I need to filter small volumes of solutions in 96 format. Any ideas? The filter needs to be inert (so C18 or C8...
01 January 2019 6,963 4 View
I would like to test the efficiency of desalting by a gel filtration column and I decided to check the desalting efficiency by monitoring removal of a dye through absorbance. Can anyone recommend...
02 February 2018 2,938 2 View
I have 500ul of sample from where I try to precipitate proteins.The sample is 2% SDS and very dilute. The conventional chl-methanol protocol suggests that I use 1:3:1:4 volumes...
04 April 2017 8,230 1 View
I need to perform offline fractionation by HILIC. I have done offline fractionation using disposable RP columns (I have used home made columns with oligo R3 material, but Thermo sells disposable...
11 November 2016 4,233 1 View
