Hello,

I am preparing rat tibialis anterior muscle samples for flow cytometry. Occasional, a sample will develop visible clumps at some point during my cell surface antibody incubation and wash steps. The samples were strained twice (100um followed by a 40um strainer) after enzymatic digestion (Collagenase II and DNase 1). Therefore, I am confident that these clumps developed after the degestion and filtering. I have been including DNase (0.03ug/ml) in my re-suspension buffer to try to mitigate this but it does not seem to help. Does anyone have any ideas on what else I could try to fix this.

Thank you for your help,