Michael R. Deyhle

3 Questions 3 Answers 0 Followers

Questions related from Michael R. Deyhle

Why are my skeletal muscle samples developing clumps during preparation for flow cytometry?

Hello, I am preparing rat tibialis anterior muscle samples for flow cytometry. Occasional, a sample will develop visible clumps at some point during my cell surface antibody incubation and wash...

03 March 2018 5,648 3 View

How long can I store a flow cytometry sample?

Does anyone know how long a fixed sample can be stored in the dark at 4 degrees C without significant loss of quality? My samples are single cell suspensions from rat skeletal muscle and spleen....

11 November 2017 5,087 6 View

For cell isolation, what is the advantage of using Percoll Plus instead of Percoll?

I am seeking guidance from investigators with experience using Percoll density gradients to isolate cells. I am trying to determine if Percoll Plus will be better for our application compared to...

10 October 2017 4,033 1 View