Why are my astrocyte cultures contaminated with alot of microglia and opc?

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Samir Ranjan Panda

Hi Hania Elhoshy

Microglia contamination in primary astrocyte cultures is quite common if we follow the traditional method of isolating astrocytes (without the use of astrocyte isolation kits). One way to easily remove the microglia is by using Liposomal clodronate. Please refer to the article attached below.

Article Liposomal clodronate selectively eliminates microglia from p...

Thanks,

Samir

Ankita Srivastava

I agree with Samir Ranjan Panda - it is a commonly occurring phenomenon. One of the key players is the day of isolation, could you confirm how old were the pups when you did the isolation? Usually pups