Can sporozoites of Plasmodium vivax be seen under light microscope?

More Hania Ramzan's questions See All

Is there a possibility to use the water for agricultural purposes in which the algae has grown?

I want to grow algae in *Water* and then want to use that algae for different purposes and also use that leftover water for agricultural purposes. Will there be any harms for the fields by taking...

28 March 2020 757 9 View

Is it possible to get good PCR product if GC content of the primer is lower than 40%?

Is it possible to get good PCR product if GC content of forward & reverse primer is 32% & 35% respectively. Tm of forward and reverse primer is 51.1 & 53.2o C respectively?

08 December 2018 7,666 9 View

What is the best method to establish psychometric properties for a questionnaire based on 3 point rating scale?

What is the best method to establish psychometric properties for a questionnaire based on a 3 point rating scale? Is it possible to use Exploratory Factor Analysis to reduce the items in the form...

23 May 2018 4,895 10 View

MATLAB code for load flow analysis of three phase unbalanced system Backward Forward Sweep Method?

Hello, I am comparing different techniques (Current Injection (Already calculated), Newton Raphson (Already Done), and Backward-Forward Sweep (facing problem)) for load flow analysis for three...

10 April 2017 9,021 4 View

Could anyone please tell me the protocol of Malaria parasite DNA Isolation from Filter paper???

I am trying to extract malaria parasite DNA from whatman filter paper blood spot by QIamp DNA blood mini kit(Qiagen, Germany), after extraction I ran the DNA on 1.5% agarose gel but did not get...

27 January 2017 6,705 5 View

What is the standard value for transmission and transformation losses (T&T losses)?

What is the standard value for transmission and transformation losses (T&T losses)? Is there any standard observed for T&T losses around the world? Is 3.4% T&T losses acceptable by any...

22 February 2015 1,865 6 View

Titanium oxides very interesting action on fibers. Clothes attached with titanium oxide can be washed just by hanging them in air (sunshine). How?

How it acts?

10 January 2012 6,192 9 View

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

Where and how to get Research collaboration and PhD sandwich program?

I am currently carrying ou a study on Plasmodium faclciparum malaria and concomitant bacteria infection. I will need an expart to collaborate with especially in molecular epidemiology, and...

07 August 2019 9,877 1 View

How to calculate number of infected RBC in mice which is infected with plasmodium berghei?

In my experiment I have to infect 2*10^7 infected RBC equally to a group of mice equally by collecting blood from donor mice.How to calculate number of infected RBC?

04 May 2019 8,078 1 View

Can heparin tubes be used to collect blood samples for Plasmodium falciparum microscopy?

I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.

23 April 2019 8,803 4 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

21 April 2019 2,699 1 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

03 April 2019 7,533 1 View

Is there any way to quantify the amount of merozoites of Plasmodium chabaudi other than by flow cytometry?

I'm doing an interaction test between erythrocytes of mice and free merozoites of Plasmodium chabaudi. Is there any way to quantify merozoites other than by flow cytometry? I have been trying in...

03 April 2019 9,682 3 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

03 April 2019 7,585 5 View

Best protocol for mercurochrome staining of mosquito midguts?

I should isolate single Plasmodium falciparum oocysts from mosquito midguts. In order to do that, I have to stain the midguts first. My present protocol is the following 1. a drop of 1%...

17 March 2019 106 1 View

Chukwuma Stephen Ezenwanne

Yes, sporozoites of Plasmodium spp can be seen with a light microscope. However, staining via IFA is necessary.

Christian G Meyer

Yes, if extracted from the salivary glands of mosquitoes. Very difficult to demonstrate sporozoites during the short migration period to the liver in humans; possible only with sophisticated live-imaging techniques as applied in our study: Article Long-term live imaging reveals cytosolic immune responses of...