I have used published primers to detect bebasia and theileria genus from ticks. I am getting bands in first PCR but not getting bands in nested PCR. I am using 5 ul product of first PCR for nested PCR.
Why are you trying to perform a nested PCR. Are the bands in the first PCR weak? I think you should also reduce the amount of amplicons you are using in your secondary PCR (e.g 1ul). High amount of PCR might be an inhibitor of your nested PCR.
Like indicated by Amzati and others, you do nested PCR when you suspect that you have very few copies of a gene that results in very few copies that cannot be detected even after the primary amplification. And thats why you will usually not see bands with the primary PCR. If you can detect strong bands with the first PCR, it means you are getting enough PCR products to be seen on Agarose gel, it may not be necessary to do a nested reaction. Otherwise use a small concentration of the primary product in the nested reaction
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