Hello! I am culturing ARPE-19 cells (passage 25) in DMEM + Sodium Pyruvate, Glutamine, 1% FBS and observing this phenotype in my cultures. These have been in culture for about 2 months and are seeded on Nunclon coated 12-well plates. Has anyone seen this odd fibrotic phenotype before, or know what might be causing it? Thank you!

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