JC-1 dye monomers are excited at lower wavelengths and emit green, while the aggregates are excited at a higher wavelength and emit red. Why is this? Why do aggregates absorb at a higher wavelength? I would only expect a greater green intensity, for aggregates, rather than a different color altogether. Why do we see this?
According to the v ery good answers of Peter and Gert, please give us folowing infos .
Why do you want to investigate this VERY OLD CYANINE DYE und very good investigated cyanine dye????
Source about JC-1
J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential
Martin Reers, Thomas W. Smith, Lan Bo Chen
Biochemistry, 1991, 30 (18), pp 4480–4486
DOI: 10.1021/bi00232a015
Publication Date: May 1991
Do you want to investigate the physical chemistry e.g. spectrocopy of this cyanine dye
in solution e.g. CUVETTES or in vivo with CELLS.
On other part it seems that you are not familiar with internet searching. Please start Google Scholar (NOT GOOGLE) and give your question :
Why are JC1 dye monomers and dimers excited at two different wavelengths?
(copy and paste) in the mask. (ca. 322 hits)
P.e. the patent of Lan B. Chen is very useful : see following link.
https://www.google.com/patents/US5169788
Concerning the answer of Gert and his pdf, please read the abstract and try to obtain the book via uni library:
http://link.springer.com/chapter/10.1007/978-3-642-04701-5_4#page-1
Abstract
J-aggregates are stable fluorescent structures formed by a number of small molecules noncovalently. These structures can be formed not only in solution but also on biological objects that act as templates. As association of these small molecules leads to drastic changes in their spectral properties, this phenomenon can in principle be used for the detection of biological structures that induce the aggregation. Here, the photophysical properties of J-aggregates formed in different media and, particularly on attachment to the objects of biological origin, such as nucleic acids, proteins, and biomembranes, are discussed.
Graphical Abstract
JRG
Thank you all and ESPECIALLY, Gert Van der Zwan! I have now got a clear enough working understanding about the properties of the chemical that I'm working with. Thank you again!
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