Why are changes in radioligand binding in imaging studies reflecting post-synaptic receptors and not pre-synaptic receptors?

23 July 2013 0 7K Report

I am reading different PET studies and came across the remark that changes in radioligand binding often reflect changes in post-synaptic receptors. Why is that? I read these in studies by Nora Volkow using raclopride in detoxified alcohol dependent individuals. Is this the same in autoradiography animal studies? Is this ligand dependent? In our animal models we are using RT-PCR to look at receptor expression. Is this more reflecting the whole pool (pre- and post-synaptic receptors and internalized ones) and is it true that the pre-synaptic receptors are many more, so possible changes in pre-synaptic receptors would be not detectable with this method?

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