Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Best way to weigh hundreds of samples for a hyb ELISA?

We use a hybridization ELISA as a PK assay. We have hundreds of samples from different tissues of various treatments. Currently we're pre-weighing the tubes, then weighing the tubes with tissue...

16 August 2018 2,261 3 View

Does anybody know about a good mouse FPR2/FPRL1 antibody for flow cytometry?

24 May 2016 2,899 1 View

Bacterial RNA of stationary phase produces very distinct CT?

I am quite new to real time PCR, so maybe I don't see my fault. I isolated RNA of bacterial cell culture of stationary phase and transcripted it to cDNA. The RNA looks fine on the gel. When I do...

20 October 2013 4,824 1 View

Does anybody know where we could buy xylan from oat spelt?

I can't find any company still selling it and we desperately need it. It's needed for different screening approaches used in our department, so it's a rather important compound to us. Any help...

02 April 2013 3,962 19 View

I want to do 2D preparative thin layer chromatography and stain with iod for detection. How likely is the probability that my lipids will oxidize?

For development I use a mixture of CHCl3, MeOH and H2O.

25 February 2013 1,261 2 View

Does Coomassie stain substances with just one or few rather primary amino groups?

Substance is stainable with Ninhydrin, but does not appear on SDS when stained with very sensitive coomassie staining.

06 February 2013 5,152 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View