Hi Ryan,

Unfortunately I don't have an answer to your question, but I would suggest to go to Nanopore community site (where you should have you profile if you bought the MinION) (https://community.nanoporetech.com/) and raise a new topic for discussion. Folks out there are pretty responsive and will probably be able to help you.

Deni

Hi Deni,

Thanks for the advice, I will likely create a post. I came here first because I find the Nanopore community site to be unnecessarily awkward and difficult considering the lack of the ability to search for topics/posts.

Cheers,

Ryan

Hi Ryan,

I would be great if you could keep this post updated once you have found the explanation for this behavior, e.g., in the Nanopore Community.

TIA.

Best,

Cedric

In general it decides based on the average q-score of the read. I think at the moment average_q > 7 is "pass", below is "fail".

One possibility is calibration strand, but I thought if detected as calibration it should pass to a calibration strand folder. I am trying to remember if albacore did calibration strand detection as default, and if it was by default it passed these reads separate to fail and pass.

Anything in the workspace should be base called so is the 32 reads in fastq format?