I have made WB using standard protocol. My protein of interest is 72 kDa. On the PONCEAU are some strange little aggregates on that weight. What may be the cause? My loading control (GAPDH) is not very good but looks much better.
There are several things which can go wrong in WB, your question does not provide details because of which it is difficult to provide concrete answer/suggestion. Only suggestion which I can think of is - You may redo it where you can load half of the amount that you loaded last time with empty lanes in between samples