Why are my cells (RAW 264.7) are not adhering on culture plates when I was reviving the cell?

I had frozen my RAW 264.7 cells from T-75 flask having 80-85% confluency, washed with 3ml PBS respectively. After washing, I added 3ml of fresh media and scrapped all the cells (also, could we use...

02 October 2018 4,768 5 View