Hi
I am looking for protein protein inteactions between a shuttle protein and nuclear hormone receptor using BIFC. When I perform BIFC with Fos and Jun I get bright signal and when I use Mutated Fos and Jun I get no signal, which indicates that my experiment is working correctly.
When I perform BIFC with my proteins of interest I get positive signal. When I perform with my nuclear receptor and Jun, I get no signal (good) but when I perform with my protein of interest and Fos I get a similar signal as my test samples.
Does anyone know the reason why this might be? The mfos/ jun combination suggests that it is not due to collisional interactions and so I am stumped as to how to approach this.
Many thanks for any assistance you can offer
To me this sentence
"when I perform with my protein of interest and Fos I get a similar signal as my test samples"
indicates that you have discovered a novel interaction, go ahead to confirm and publish
good luck
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