Hi!

Recently started using a new genotyping kit for PCR and all of a sudden started seeing non-specific bands on the gel. At first I thought contamination, but I've tried all new primers and master mix three different times and still see the same results. Also, all of the products seem to have positive signals. The negative also shows a positive band. However, when I use my old genotyping kit this same negative does not show a signal. Even my NTC (no template control) is showing non-specific bands, but no signal. Any help is greatly appreciated, thanks!

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