There is no smear. RNA is having high concentration and quality (nanodrop).what may be the reason?
what is the reason for single band for RNA after AGE? I used TRIZOL method for RNA isolation
Would very much agree with Tiago Oliveira above. I was facing some problems with RNA isolation and would like to add that you examine the curves from the nanodrop before you run it on the gel. Good curves on nanodrop should look good on denaturing gel. All the best!
Did you try DNAse treatment after isolation?. Just to be sure there are no DNA traces in your samples. Also as Tiago already sugested denaturing electroporesis or maybe increasing the concentration of your AGE may improve resolution of your bands.
Dear Manu,
Load about 500ng of denatured (in RNA loading buffer containing formamide) on 1% agarose gels prepared in MOPS buffer. On the other hand try to interpret the nanodrop readings and curves according to the the following article
https://www.promega.in/resources/pubhub/methods-of-rna-quality-assessment/
All the best
run a Formaldehyde agarose RNA gel, TRizol should give u a good RNA.. if still the problem persists maybe there could be DNA contamination.. did you do DNAse treatment
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