Manu Prasad

10 Questions 17 Answers 0 Followers

Questions related from Manu Prasad

Which is the best method to isolate single cells from mice tumor tissues for Sc RNA sequencing?

Can someone suggest a best method to isolate single cells suspension for ScRNA sequencing with out tissue debris and maximum viability.

27 April 2022 8,693 0 View

Flow cytometry protocol for pERK staining?

Can somebody send me a protocol for intracellular staining of pERK in spleen and tumor cells?

23 October 2019 1,145 1 View

Markers for dendritic cells and macrophages?

How can we differentiate between dendritic cells and macrophages? What are the population that express cd11c and cd11b and mhc class 11 and gr1-

29 June 2019 5,841 4 View

AAV9 virus purification

Which is the easiest and cheap method to purify adenovirus, AAV9. Has any one experience?

20 July 2017 5,999 5 View

After tamoxifen induction (5 DAYS IP) cre recombinase is not trans locating to the nucleus.

I HAVE KRT14 CRE MICE (CRE-ert).TAMOXIFEN INDUCIBLE.

03 July 2017 5,377 7 View

How can we avoid autofluorescence produced by RBC in paraffin embeded tissue sections during immunofluorescence?

RBCs trapped inside tssue (colon) sections (PFA fixed) showing fluorescence while doing confocal immunofluorescence analysis

29 January 2016 4,023 3 View

Why am I getting a single thick band after running RNA?

There is no smear. RNA is having high concentration and quality (nanodrop).what may be the reason? what is the reason for single band for RNA after AGE? I used TRIZOL method for RNA isolation

24 April 2015 9,080 5 View

Is there any relationship between polyploidy and drug resistance in cancer ?

Polyploidy and drug resistance

18 February 2015 7,186 6 View

How do you find transcription factors of a particular gene?

Transcription factor finding

28 January 2015 2,847 3 View

How do I develop a 5FU resistant cancer cell line?

I want to make 5FU resistant cancer cell line. please give your valuable suggestions.

16 January 2015 2,974 2 View