I continually get peaks in my no enzyme control for real time PCR in my melt curve. Though, my Cts are ~7 cycles away. I have DNase treated my RNA twice, changed all reagents and decontaminated pipettes as well as worked at a different space. Any idea why this is happening?
Beth Caruana
Hi Genghis
In my NEC there is RNA, primers, SYBR and water. I've run the products on a gel and they are at the same size as my intended product so I'm quite confident that they arn't primer dimers
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Beth Caruana
Ive used both sealed plates and tubes on the Vii47 and rotor gene respectively.
Also I've separated the NEC and NTC from my samples to prevent contamination to no avail.
At this point im just going to start from scratch and hope for the best
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