The target transcript was clearly detected in my Northern blot results. Howerer, two " white" bands which are the same band sizes and locations as 18s and 28S rRNA were shown, what's the reason for this? thanks in advance.
don't worry, this happen when there is a lot of material in one position (here 18 and 28S): there is no room for the probe to hybridize so there is no background at this position ... hence the white bands. It happens also with proteins in western blot ....
ribosomal RNA are so abundant that they exclude all other RNA within the band and therefore exclude the ones that might weakly hybridize with your probe, thereby reducing the background on the band. This just means the ribosomal RNA do not hybridize with your probe at all. Not a problem at all
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