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Preethidan DS

You can try with combinations of Chloroform/methanol for the separation of sequiterpene lactones. Also refer the two papers attached

Best,

DSP

Frederick Crane

sorry i dont the answer F Crane

Neeraj K. Sethiya

Absorbents for TLC: Merck RP 18 F254s; Merck silica gel 60 F254s;

Solvents for TLC: mixt, of CH2C12 with MeOH; mixt, of cyclohexane with

EtOAc.

Detection on TLC-plates: UV-light, spray reagent (anisaldehyde-H2S 0 4 on silica gel)

Please refer these papers.