Organic solvent for trehalose?

I am looking for an organic solvent for trehalose. DMSO works, methanol works, like ethanol, but both are not suited for the rest of my process. Any ideas or exprience here?

04 August 2020 6,984 3 View

Elution of an antibody on a peptide immobilized column did not work?

I am very disappointed. I coupled a peptide with an N-terminal cysteine on a sulfolink column. HPLC shows that the peptide immobilization was almost complete. After cyclisation of the serum the...

25 May 2020 1,265 8 View

Hi! Can anyone show me how to calculate the total anthocyanins content using the OD at 545, 535 and 515 nm?

27 January 2017 7,419 3 View

Would you suggest any software executing ANN (MLP type) where connections between neurons (in consecutive layers) can be separately defined by user?

10 October 2016 3,464 7 View

What are the specific possible biases involved in having one coder in a qualitative analysis?

27 June 2016 998 4 View

Is there a reference article documenting the preferred imaging modes in oncology?

I would like citing a reference in a manuscript about the most used imaging modalities in oncology. Did somebody could provide me with a pointer?

17 August 2015 5,791 3 View

What is a clear definition for radiomics?

I am looking for a clear definition of "Radiomic". According to different papers from: Kuo, 2007; Lambin. 2012; aerts. 2014; singanamalli, 2014 and some others, I am not sure if the concept of...

15 February 2015 6,726 8 View

How can I compute the limit of agreement with Bland Altman featuring heteroscedasticity?

I am analysing repeated measurement of ratio-scaled data. For doing that I rely on Bland Altman analysis. The common case where Limit of Agreement is horizontal can be adressed by most of the...

20 February 2014 4,709 2 View

How to manage Bland Altman analysis with more than one couple of measurements?

I consider three readers circularly involved in Bland-Altman 2-by-2; threfore I obtained 3 Bland-Altman analysis with corresponding Limit of Agreement (and Confidence Interval). In order to get...

16 January 2014 9,164 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to formulate Energy minimization in Molecular dynamics simulation for alloys using meam potential?

during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...

22 February 2021 380 5 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View