Who knows a good surgical monitoring system for mice?

Kay-Dietrich Wagner @Kay-Dietrich_Wagner

03 March 2019 1 5K Report

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a alternative with a more reasonable price?

Kay-Dietrich Wagner

Dear Jef,

thank you. This one I know. Unfortunately seems their is no real competitor on the market.

Badges
Science topic

More Kay-Dietrich Wagner's questions See All

ANSYS FLUENT is only launching in parallel. Can somebody please help?

My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!

04 September 2020 4,199 4 View

I am trying to simulate an electric arc formed because of current flowing through air between 2 conductors in ANSYS Fluent. Can i use MHD module?

If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...

15 August 2020 200 1 View

Removal of dead cells before harvesting for Proteomics?

Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...

03 March 2020 2,329 3 View

Enrichment of AHA-tagged proteins: Should I use DBCO- or Alkyne-based methods?

Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...

18 February 2020 8,725 2 View

Why do you need to preheat antigen retrieval buffer?

I'm troubleshooting immunofluorescence staining for some antibodies. Some work fine with my protocol [H2AX, Ki67, 3-nitrotyrosine], some stained once and never again [8oxoG], and some won't stain...

21 April 2019 5,510 3 View

What is the ideal activation time for stain-free gels?

From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...

25 March 2019 1,508 4 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

28 February 2019 2,000 1 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

11 December 2018 7,269 3 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

02 December 2018 7,486 3 View

What statistical analysis is recommended to compare profiles?

I am looking to compare two cultural groups (independent variable) and their resilience profile (dependent variable). There are six factors within the profile, so I feel that a MANOVA would be...

14 November 2018 8,566 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View