How to maintain and expanse brain tumor-derived lymphocytes in vitro?

Hi everyone, I'm trying to isolate lymphocytes from tumors resections (GBM) and to maintain them in culture. I would like also to expanse them to use for several experiments. Does someone has...

10 November 2017 9,370 4 View

Does it exist an application for in vivo follow up?

07 August 2017 4,138 2 View

Why Wrist ankle do not exist for mouse identification ?

03 April 2017 330 5 View

Do we need Matrigel for SW48 colon cancer cells SC-injection?

07 August 2016 9,063 4 View

Why NOD-Scid do not tolerate irradiation while nude mice do ?

05 June 2016 8,175 6 View

IPTG or Doxycycline in vivo? What's better ?

Hello everyone, I wondered what's the advantage to use IPTG in place of Doxycycline for gene expression modulation in vivo ? I already eared that DOX can become be toxic for mice in case of long...

11 December 2015 8,185 8 View

Is there an in silico data available for EGFR V3 mutation in GBM patients ?

Hello everyone, I would like to find a database available on Internet to select a population of GBM patients who display the EGFR V3 mutation. An in this cohort of patients, I would like also to...

09 October 2015 2,303 7 View

How do I perform in vitro irradiation on GBM cells?

03 April 2015 1,096 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View