How to maintain and expanse brain tumor-derived lymphocytes in vitro?

Hi everyone, I'm trying to isolate lymphocytes from tumors resections (GBM) and to maintain them in culture. I would like also to expanse them to use for several experiments. Does someone has...

10 November 2017 9,370 4 View

Does it exist an application for in vivo follow up?

07 August 2017 4,138 2 View

Who is using RapID system for mouse identification?

07 August 2017 2,032 1 View

Do we need Matrigel for SW48 colon cancer cells SC-injection?

07 August 2016 9,063 4 View

Why NOD-Scid do not tolerate irradiation while nude mice do ?

05 June 2016 8,175 6 View

IPTG or Doxycycline in vivo? What's better ?

Hello everyone, I wondered what's the advantage to use IPTG in place of Doxycycline for gene expression modulation in vivo ? I already eared that DOX can become be toxic for mice in case of long...

11 December 2015 8,185 8 View

Is there an in silico data available for EGFR V3 mutation in GBM patients ?

Hello everyone, I would like to find a database available on Internet to select a population of GBM patients who display the EGFR V3 mutation. An in this cohort of patients, I would like also to...

09 October 2015 2,303 7 View

How do I perform in vitro irradiation on GBM cells?

03 April 2015 1,096 4 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Do you have papers on hindlimbs myology of birds to share?

Hi all! My work team and I are looking for papers on muscular descriptions of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than...

22 February 2021 8,330 6 View

Does someone know which structure could exit the olfactory bulbs at their antero-dorsal top ?

Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...

27 January 2021 797 6 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

Why does Reaction Wood acts opposite in gymnosperm and angiosperms?

I am wondering why the Gymnosperms have an opposite behaviour that Angiosperms in the Reaction Wood (RW). Are there some new findings regarding that? Studies say that causal agents can be the...

10 January 2021 8,744 4 View

Could you recommend a book that has change your view of seeing kinesiology (sport science, human anatomy & physiology) in a different matter?

I'm looking for something inspirational which would show me a different side of kinesiology. Something easy to understand but also educational.

26 November 2020 4,611 10 View

Has anyone seen this perivascular black stuff in rat brains?

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

19 November 2020 8,108 4 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

28 October 2020 7,768 3 View

How Selenometheonine helps on the growth of bMECs (bovine mammary epithelial cells) under stress conditions?

I'm searching for articles related to "How Selenometheonine helps on the growth of bMECs (bovine mammary epithelial cells) under stress conditions?". Anyone can help?

08 October 2020 8,431 3 View